Journal: PLoS ONE

Article Title: Autoimmunity in CD73/Ecto-5′-Nucleotidase Deficient Mice Induces Renal Injury

doi: 10.1371/journal.pone.0037100

Figure Lengend Snippet: (A) Using immunohistochemistry with a CD73-specific antibody and a rhodamin red λ conjugated secondary antibody, confocal microscopy at a magnitude of 10× revealed staining of glomerular cells in the mesangium as well as peritubular cells in renal tissue of WT mice which is absent in the mutant. Images are representative of 3 of 5 animals with more than 2 sections per kidney. (B) Renal function estimated by the measurement of serum creatinine, auto normalized, in WT and CD73 −/− mice. 8 to19 mice per group were used. Data are presented as means ± SEM, level of significance as indicated according to Student t-test. (C) Total proteinuria was elevated and increased with age in CD73 −/− mice as compared to the wild type controls. We detected proteinuria/creatinuria in [g/g], left panel, and total urine protein in [mg/24h], right panel. 8 to 19 mice per group were used. Data are presented as means ± SEM, level of significance as indicated according to Student t-test.

Article Snippet: The following secondary antibodies were used as appropriate: fluorescein isothiocyanate (FITC)-conjugated anti-rat polyclonal Ab (goat, Dianova, Hamburg, Germany), rhodamine red λ conjugated anti-rat polyclonal Ab (goat, Dianova, Hamburg, Germany), Cy TM 3 labeled anti-goat Ab and anti-rabbit Ab (donkey, Dianova, Hamburg, Germany).

Techniques: Immunohistochemistry, Confocal Microscopy, Staining, Mutagenesis

Journal: PLoS ONE

Article Title: Autoimmunity in CD73/Ecto-5′-Nucleotidase Deficient Mice Induces Renal Injury

doi: 10.1371/journal.pone.0037100

Figure Lengend Snippet: The renal cortex of CD73 −/− mice showed glomerular deposits of IgG and C3 as well as increased presence of CD 11b- a n d CD8- positive cells as well as CD25- and GR-1–positive cells in the interstitium of CD73 −/− mutants which were absent in WT-mice. (A) IgG staining (green, FITC conjugated secondary antibodies) and C3 staining (red, rhodamin red λ conjugated secondary antibodies) was carried out in cryo-conserved, 7-µm sections of the kidneys of WT and CD73 −/− mice. Images are representative of >3 independent experiments. (B) CD11b and CD 8 staining (green, FITC conjugated secondary antibodies) and CD25 and GR-1 staining (red, Cy 3) was performed in cryo-conserved, 6–7-µm sections of the mice kidneys. Images are representative of 5 to 7 animals with more than 2 sections per kidney.

Article Snippet: The following secondary antibodies were used as appropriate: fluorescein isothiocyanate (FITC)-conjugated anti-rat polyclonal Ab (goat, Dianova, Hamburg, Germany), rhodamine red λ conjugated anti-rat polyclonal Ab (goat, Dianova, Hamburg, Germany), Cy TM 3 labeled anti-goat Ab and anti-rabbit Ab (donkey, Dianova, Hamburg, Germany).

Techniques: Staining